INDUKSI KALUS EMBRIOGENIK BEBERAPA GENOTIPE UBI KAYU (Manihot esculenta Crantz.)
Abstract
Somatic embryogenesis is one of the important steps in the in vitro propagation and crop improvement of cassava. The experiment was aimed to induce the formation of somatic embryos from immature leaves, shoot tips, and petioles explants on eight types of embryogenic callus induction medium. The research consisted of four experiments, each using UJ 5, Jame-jame, Gajah, and Adira 4 genotypes as explants. The results showed that immature leaves and shoot tips cultured on the MS + 20 g L-1 sucrose + 8 mg L-1 2.4-D medium and MS + 20 g L-1 sucrose + 10 mg L-1 NAA medium showed the highest numberand percentage ofexplantsformingcallus, fastest initiation of callus, highestcallusgrowth,and highest diameter of callus.
Key word : Cassava, 2.4-D, NAA, somatic embryogenesis
References
Atehnkeng, J., V.O. Adetimirin, S.Y.C. Ng. 2006. Exploring the African cassava (Manihot esculenta Crantz.) germplasm for somatic embryogenic competence. Afr J Biotechnol. 5(14):1324-1329.
Fletcher, E.K.A., T.N.E. Amoako, P. Twumasi. 2011. Effect of 2.4-D, explants type and cultivar on the callogenesis expression of cassava (Manihot esculenta Crantz.) in Ghana. Afr J Biotechnol. 10(46):9396-9401.
Guohua, M., X. Qiusheng. 2002. Induction of somatic embryogenesis and adventitious shoots from immature leaves of cassava. Plant Cell Tiss Org Culture. 70:281–288.
Hankoua, B.B., N.J. Taylor, S.Y.C. Ng, I. Fawole, J. Puonti-Kaerlas, C. Padmanabhan, J.S. Yadav, C.M. Fauquet, A.G.O. Dixon, V.N. Fondong. 2006. Production of the first transgenic cassava in Africa via direct shoot organogenesis from friable embryogenic calli and germination of maturing somatic embryos. Afr J Biotechnol. 5(19):1700-1712.
Ibaraki, Y., K. Murata. 2001. Automation of somatic embryo production. Plant Cell Tiss Org Culture. 65(3):179-199.
Ibrahim, A.B., F.F. Heredia, C.B. Pinheiro, F.J.L. Aragao, F.A.P. Campos. 2008. Optimization of somatic embryogenesis and selection regimes for particle bombardment of friable embryogenic callus and somatic cotyledons of cassava (Manihot esculenta Crantz.). Afr J Biotechnol. 7(16):2790-2797.
Joseph, T., H.H. Yeoh, C.S. Loh. 2000. Somatic embryogenesis, plant regeneration and cyanogenesis in Manihot glaziovii Muell. Arg. (ceara rubber). Plant Cell Rep. 19:535-538.
Opabode, J.T., O.O. Oyelakin, O.A. Akinyemiju, I.L. Ingelbrecht. 2014. Influence of type and age of primary somatic embryo on secondary and cyclic somatic embryogenesis of cassava (Manihot esculenta Crantz.). Br Biotechnol J.4(3):254-269.
Osorio, M., E. Gamez, D. Infante. 2012. Evaluation of cassava plants generated by somatic embryogenesis in different stages of development using molecular markers. Electron J Biotechnol. 15:1-11.
Priadi, D., E. Sudarmonowati. 2006. Pengaruh komposisi media dan ukuran eksplan terhadap pembentukan kalus embriogenik beberapa genotip lokal ubi kayu (Manihot sculenta Crantz.). Biodiversitas. 7(3):269-272.
Rossin, C.B., M.E.C. Rey. 2011. Effect of explants source and auxins on somatic embryogenesis of selected cassava (Mannihot esculenta Crantz) cultivar. S Afr J Bot. 77:59-65.
Sudarmonowati, E., G.G. Henshaw. 1996. The use of picloram and dicamba to induce somatic embryogenesis in cassava. Ann Bogor. 4(1):27-34.
Szabados, L., R. Hoyos, W. Roca. 1987. In vitro somatic embryogenesis and plant regeneration of cassava. Plant Cell Rep. 6 (3):248-251.
Taylor, N.J., M.V. Masona, R. Carcamo, T. Ho, C. Schöpke, C.M. Fauquet. 2001. Production of embryogenic tissues and regeneration of transgenic plants in cassava (Manihot esculenta Crantz.). Euphytica. 120:25-34.
Thro, A.M., W.M. Roca, J. Restrepo, H. Caballero, S. Poats, R. Escobar, G. Mafla, C. Hernandez. 1999. Can in vitro biology have farm-level impact for small-scale cassava farmers in latin America. In Vitro Cell Dev Biol Plant. 35 (5):382-387.
Uzelac, B., Ninkovic, A. Smigocki, S. Budimir. 2007. Origin and development of secondary somatic embryos in transformed embryogenic cultures of Medicago sativa. Biol Plant. 51(1)1-6.
Vidal, A.M., M.A.P.C. Costa, A.S. Souza, W.A.B. Almeida, F.V.D. Souza. 2014. In vitro regeneration and morphogenesis of somatic embryos of cassava. Rev Ciencia Agronom. 45(3):558-565.
Walpole, R.E. 1995. Pengantar statistika ed ke-3. PT. Gramedia Pustaka Utama, Jakarta.
Wongtiem, P., D. Courtois, B. Florin, M. Juchaux, D. Peltier, P. Broun, J.P. Ducos. 2011. Effects of cytokinins on secondary somatic embryogenesis of selected clone Rayong 9 of Manihot esculenta Crantz for ethanol production. Afr J Biotechnol. 10(9):1600-1608.
